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. 2014 Jan 31;192(5):2252–2260. doi: 10.4049/jimmunol.1301249

FIGURE 5.

FIGURE 5.

NO release. (A) Raji or WIL2S cells incubated with human M1 or M2c macrophages (E:T ratio, 1:1) for 24 or 48 h in the presence of 1 μg/ml GA101 GE or WT Abs and ofatumumab. NO release in culture supernatants was assessed with Griess reagent and corresponds to the average of pooled triplicates derived from three independent experiments (two using Raji and one using WIL2S as targets). (B) Raji cells incubated with human classical or CD16+ monocytes (E:T ratio, 10:1) for 22 h in the presence of 1 μg/ml GA101 GE or WT Abs. NO release in culture supernatants was assessed by Griess reagent using pooled quadruplicates derived from one experiment. Statistical analysis, unpaired t test: *p < 0.05, **p < 0.01, ***p < 0.001.