Table II. The percentages of circulating lymphocytes, monocytes, and granulocytes following neutrophil depletion.
| Gr-1 Depletion |
Ly-6G Depletion |
|||
|---|---|---|---|---|
| IgG + LPS (n = 6) | Anti–Gr-1 + LPS (n = 7) | IgG + LPS (n = 7) | Anti–Ly-6G + LPS (n = 7) | |
| Percent of circulating lymphocytes/total leukocytes | 35.65 ± 1.78 | 71.41 ± 5.34 | 33.89 ± 3.07 | 58.9 ± 4.64 |
| Percent of circulating monocytes/total leukocytes | 7.37 ± 0.59 | 10.93 ± 1.91 | 9.35 ± 0.45 | 20.24 ± 4.08 |
| Percent of circulating granulocytes/total leukocytes | 54.73 ± 2.46 | 14.37 ± 5.34 | 55.2 ± 3.20 | 16.69 ± 4.19 |
Data presented as mean ± SEM. Flow cytometric analysis of maternal blood collected 6 h post–LPS administration from mice treated with anti–Gr-1, anti–Ly-6G, or the appropriate isotype control Ab, prior to intrauterine LPS. Lymphocytes, monocytes, and granulocytes were gated based on their forward and side scatter profiles, and the proportion of circulating granulocytes, monocytes, and lymphocytes as a percentage of the total leukocytes was calculated.