. Author manuscript; available in PMC: 2014 Dec 31.

Published in final edited form as: Biochemistry. 2013 Dec 17;52(52):9413–9425. doi: 10.1021/bi4010304

Table 1.

Parameters for Simulations. Most of the composite data were obtained from the simulated percentages of MB spectra in Figures 3 and 4. Composite data of YPAD cells at early exponential growth mode were estimated by assuming that the Fe distribution in the cells was identical to that in the YPAD cells at late exponential growth mode.

	[Fe_cell]	[Fe_C]	[Fe_M]	[Fe_P]	[Fe_V]	k_v (hr⁻¹)	k_M (hr⁻¹)	α (hr⁻¹)

YPAD, Early Exp.	300_D	24_D	40_D	0_D	236_D	4.8	0.8	0.65
	314_C	32_C	40_C	5_C	234_C
	310_V	32_V	40_V	1_V	237_V

YPAD, Late Exp	700_D	56_D	90_D	0_D	550_D	4.8	0.8	0.29
	677_C	33_C	89_C	11_C	553_C
	667_V	33_V	84_V	7_V	543_V

YPAD, Slow-growth	1500_D	75_D	150_D	285_D	990_D	0.43	0.60	0.034
	1577_C	79_C	150_C	288_C	986_C
	1359_V	90_V	152_V	128_V	989_V

MM, Exponential	430_D	13_D	90_D	0_D	327_D	5.2	1.6	0.50
	454_C	31_C	89_C	6_C	327_C
	450_V	32_V	89_V	2_V	327_V

MM, Stationary 24 hrs	1500_D	180_D	135_D	165_D	1020_D	0.90	0.16	0.00
	1367_C	61_C	132_C	147_C	1027_C
	1284_V	65_V	135_V	40_V	1044_V

MM, Stationary 120 hrs	8100_D	170_D	130_D	2000_D	5800_D	0.90	0.16	0.00
	7698_C	61_C	185_C	1173_C	6279_C
	6993_V	65_V	187_V	2077_V	4664_V

Subscripts D = composite data, V = version V, and C = version C. Concentrations are in μM. Parameters: R_HI, 330 μM/hr; R_LO, 30 μM/hr; K_C, 30 μM; cs, 3; K_M, 115 μM; ms, 8. For version C, px = 1.4 and k_P = 0.034 hr⁻¹. For version V, px = 1.3 and k_P = 0.0006 hr⁻¹.