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. 2014 Feb 5;111(7):2794–2799. doi: 10.1073/pnas.1308758111

Fig. 3.

Fig. 3.

Activation of Sox6 expression by Sox2 in vivo. (A) Expression constructs are schematically illustrated. cSox2-myc: Sox2 with a myc-epitope tag (dark gray) at the C terminus. HMG-VP16: The HMG domain of Sox2 fused to the transactivation domain of VP16 (green) and nuclear localization signal (yellow) at the N terminus. HMG-EnR: The HMG domain of Sox2 fused to the repressor domain of Engrailed (purple) with a myc-epitope tag (dark gray) at the C terminus. (B–G) Electroporated chicken embryos were harvested 30–48 h after electroporation and processed for immunohistochemical staining and RNA in situ hybridization on serial sections. Expression of constructs was confirmed by immunostaining for the myc epitope or VP16 (B, D, and F). Forced expression of Sox2 (B and C) or HMG-VP16 (D and E) promoted the expression of Sox6, but forced expression of HMG-EnR (F and G) suppressed the expression of Sox6. Adjacent sections at the forelimb level were used.