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. 2014 Feb 3;111(7):2482–2487. doi: 10.1073/pnas.1400283111

Fig. 1.

Fig. 1.

The binding characteristics of mAb MC813-70 to GBM cell lines. (A) Schematic diagram of the biosynthesis of globo-series GSLs. SSEA-3, the precursor of SSEA-4 and Globo H, is synthesized from globoside. Glycosidic linkages and graphic notations are labeled. Glc, glucose; Gal, galactose; GalNAc, N-acetylgalactosamine; Fuc, fucose; NeuAc, N-acetylneuraminic acid. (B) GBM cells were stained with Alexa Fluor 488-conjugated MC813-70, and the staining intensity was analyzed with flow cytometry. All cells examined were GBM cell lines except for SVG p12, which is a normal human fetal glial cell line transformed with SV40 large T antigen. The histograms of the cells stained with MC813-70 and isotype control are shown in gray and white, respectively.