Fig. 5.

Mutational analysis of the ctNup53 interactions with ctNup192 and Kap-α. (A) Sequence comparison of ctNup53^31–67 with a consensus classical bipartite NLS and summary of the effects of ctNup53^31–67 mutations on ctNup192^NTD and Kap-α binding. Basic residues are highlighted in blue, and dots indicate mutations that cause no effect (green), reduced binding (orange), or complete disruption (purple) (Fig. S6 A and B). (B) Mutational analysis of the ctNup192^NTD–ctNup53^31–67 interaction. Mutations that have no effect (−, black), reduce binding (+, orange), or abolish the interaction (+++, red) are indicated (Fig. S7A). (C, Right) Surface mapping of ctNup192^NTD mutagenesis results. (C, Left) As a reference, a cartoon representation of ctNup192^NTD, colored as in Fig. 1B, is shown. The locations of mutations on the ctNup192^NTD surface are labeled and colored in green, orange, and red to indicate no effect, reduced binding, or complete disruption of ctNup53^31–67 binding, respectively.