Fig. 1.

Growth of R. pomeroyi DSS-3 on TMA and TMAO as a sole N source. R. pomeroyi DSS-3 was grown on either TMA (white circles) or TMAO (gray circles) and concentrations of TMA (white diamonds) and TMAO (gray diamonds) were quantified throughout the growth. The function of tdm was determined by comparing growth of the wild type (A) against the Δtdm::Gm mutant (B). When the mutant was corrected with a native tdm from either R. pomeroyi DSS-3 (D) or Pelagibacteraceae strain HIMB59 (E) growth was restored, whereas the vector control (pBBR1MCS-km) did not restore the growth of the mutant on TMA or TMAO (C). All cultures were grown in triplicate and error bars denote SDs.