Fig. 2.

FLNA increases the HIF-α transactivation function. M2 and A7 cells were cultured under normoxic (N) or hypoxic (H) conditions. (A and B) HRE-driven luciferase reporter activity (A) and expression of HIF target genes (B) are higher in A7 cells compared with M2 cells. (C) A7 cells cultured in normoxia or hypoxia secrete more VEGF-A than M2 cells. (D and E) Silencing of FLNA reduces HIF target gene expression (D) and VEGF-A secretion (E) in A7 cells in both normoxia and hypoxia. A7 cells were transfected with a negative control siRNA (CTR) or a siRNA targeting FLNA (FLNA). Data are shown as mean ± SD of at least three independent experiments. *P < 0.05 compared with corresponding samples in M2 cells; ^#P < 0.05 compared with corresponding samples transfected with control siRNA.