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. 2014 Feb 3;111(7):2560–2565. doi: 10.1073/pnas.1320815111

Fig. 3.

Fig. 3.

FLNA interacts with HIF-1α but not with HIF-2α. (A) FLAG-fused HIF-1α interacts with HA-tagged FLNA repeats 20–24 (HA-FLNA20-24) in NIH 3T3 cells. (B) Endogenous FLNA and HIF-1α interact in the cytoplasmic compartment. Nuclear (Nuc) and cytosolic (Cyt) extracts were prepared from COS1 cells cultured in normoxia (N) or hypoxia (H). (C) Identification of HIF-1α domains interacting with FLNA. HEK 293A cells were cotransfected with plasmids encoding HA-FLNa20-24 and FLAG-mHIF-1α (1-390), (392-622), (531-822), or pCMX as control. (D) HA-FLNA20-24 interacts with HIF-1α but not with HIF-2α. HEK 293A cells were cotransfected with plasmids encoding HA-FLNA20-24 and FLAG-mHIF-1α, FLAG-mHIF-2α, or pCMX as control. *Unspecific signal.