Fig. 7.

FLNA is recruited to promoters of HIF-1α target genes at hypoxia. M2, A7 cells (A), and COS-1 cells (B) were cultured at normoxia (N) or hypoxia (H) for 8 h. Soluble chromatin was immunoprecipitated using antibodies against HIF-1α or the C terminus of FLNA, or control IgG, and analyzed by PCR using primers spanning HRE-containing regions of VEGF-A or PGK1 promoters. (A) Both HIF-1α and FLNA are recruited to the HRE-containing regions of the VEGF-A promoter in FLNA-expressing A7 cells under hypoxic treatment. (B) HIF-1α and FLNA are detected on VEGF-A and PGK1 promoters in COS-1 cells cultured under hypoxic conditions.