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. 2014 Feb 3;111(7):2566–2571. doi: 10.1073/pnas.1322923111

Fig. 2.

Fig. 2.

AgOrco protein expression in spermatozoa. IHC labeling of spermatozoa with an Orco antibody. (A) Left, anti-Orco (green); Center, anti–α-tubulin (blue); Right, overlay of green and blue signals (cyan) plus propidium iodide (magenta). (B) Left, anti-Orco preincubated with AgOr18 peptide (green); Center, anti–α-tubulin (blue); Right, overlay of green and blue signals (cyan) plus propidium iodide (magenta). (C) Left, anti-Orco preincubated with Orco-specific peptide (green); Center, anti–α-tubulin (blue); Right, overlay of green and blue signals (cyan) plus propidium iodide (magenta). Scale bar in C applies to all images.