Fig. 4.

Orco function and expression in A. aegypti sperm/testes. (A) Activation indices (y axis; + SEM) at given log₁₀[M] (x axis) for wild-type (white bars) or orco^−/− mutant spermatozoa (striped bars) in response to buffer (Left), VUAA4 (Center), or cAMP (Right). n = 6 for all compound treatments and n = 7 for buffer control. (B and C) IHC labeling of A. aegypti testes. (B) Wild-type and (C) orco^−/−. Left, anti-Orco (green); Right, overlay of anti-Orco (green), anti–α-tubulin (blue), and propidium iodide (magenta) signals.