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. 2014 Feb 3;111(7):2566–2571. doi: 10.1073/pnas.1322923111

Fig. 5.

Fig. 5.

Orco protein expression in spermatozoa. IHC labeling of spermatozoa with an Orco antibody. Left, anti-Orco (green); Right, overlay of anti-Orco (green), anti–α-tubulin (blue), and propidium iodide (magenta) signals. (A) Anti-Orco labeling in D. melanogaster w1118 testes. (B) Anti-Orco labeling in D. melanogaster orco−/− testes. (C and D) Anti-Orco labeling in N. vitripennis testes without (C) or with (D) preincubated Orco-specific peptide. (E and F) Anti-Orco labeling in A. albopictus testes without (E) or with (F) preincubated Orco-specific peptide. Scale bar in B applies to all images.