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. 2014 Jan 21;111(7):2734–2739. doi: 10.1073/pnas.1308241111

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Fig. 4. — Kid uncouples DNA replication and cell division in E. coli. (A) Average numbers of oriC- (red) and oriR1- (green) foci in ILO1 cells carrying plasmids pWX6, mR1tetO₂₄₀, and pPrTs_LWCKid (Kid) and cultured at 42 °C for the indicated times. Values are normalized against those in parallel control samples carrying pWX6, mR1tetO₂₄₀, and empty pPrTs_LWC (Ctrl). Representative images of cells at different time points are shown. (B) Relative abundance of oriC-ter (OT) phenotypes observed in ILO6 cells carrying plasmids pWX6 and pPrTs_LWC (Ctrl), after culturing them at 42 °C for the indicated times. Images of representative phenotypes in this sample are shown, and the average number of oriC (green) and ter (red) foci per cell, as well as oriC/ter ratios per cell and time point, are indicated. (C) Same as in B but in cells carrying pWX6 and pPrTs_LWCKid. Average numbers of foci result from dividing the total number of each type of foci by the total number of cells (minimum of 600) analyzed per sample and time point.