Fig. 1.

Locations of DENV-2 vsRNAs in the viral genome and the effect of their inhibitors on virus replication. (A) Diagram showing the genome of DENV with locations of vsRNAs mapped to the genome. (B) RT-qPCR analysis to examine the levels of viral gRNA in Aag2 cells transfected with synthetic inhibitors of the six vsRNAs shown in A followed by DENV-2 infection at 7 dpi. ****P < 0.0001 ANOVA. (C) Virus titer determination in the medium from three replicates of the experiment described in B by using the MTT method; **P = 0.0043 ANOVA. (D) Secondary structure of the stem-loop in DENV-2 3′UTR and the location of DENV–vsRNA-5 shown with a gray shadow.