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. 2014 Feb 3;111(7):2746–2751. doi: 10.1073/pnas.1320123111

Fig. 2.

Fig. 2.

DENV–vsRNA-5 expression in Aag2 cells. (A) Northern blot analysis using a specific probe to vsRNA-5 in DENV-2–infected Aag2 cells at 3, 5, and 7 dpi; M, mock-infection. U6 RNA serves as loading control. Arrows indicate the precursor and mature vsRNA-5. (B) Northern blot analysis of vsRNA-5 in C6/36 cells infected with DENV-2 at 3, 5, and 7 dpi using the 5′ probe (Left); the same blot was used for 3′TL probe (Right). (C) Northern blot analysis for detection of vsRNA-5 in Aag2 cells transfected with 3′UTR ssRNA from DENV-2 using vsRNA-5 5′, 3′TL, and vsRNA-2 probes. SS1 and SS2 show two replicates from separate transfections. (D) miRNA RT-qPCR analysis confirming ectopic expression of vsRNA-5 in Aag2 cells transfected with pIZ/pre-5 (72 h after transfection) encoding the precursor of DENV–vsRNA-5. pIZ, empty vector. (E) miRNA RT-qPCR analysis for expression of vsRNA-5 in the mammalian Vero cells infected with DENV-2. Data were normalized against 5S rRNA.