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. 2014 Feb 3;111(7):2746–2751. doi: 10.1073/pnas.1320123111

Fig. 3.

Fig. 3.

Biogenesis of DENV–vsRNA-5 is mainly AGO2 dependent. (A) Northern blot detection of DENV sfRNA in the cytoplasmic fraction of Aag2 cells infected with DENV-2 at 7 dpi. C, cytoplasmic; M, mock infection; N, nuclear RNA fractions. To distinguish cytoplasmic and nuclear fractions, 18s rRNA and 5s rRNA are shown, respectively. (B) RT-qPCR analyses to show detection levels of vsRNA-5 in control (dsgfp), Dicer-1, Dicer-2, AGO1, and AGO2 silenced Aag2 cells infected with DENV-2 at 7 dpi. There are statistically significant differences between groups with different letters at P < 0.0001.