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. 2014 Feb 3;111(7):2746–2751. doi: 10.1073/pnas.1320123111

Fig. 5.

Fig. 5.

Validation of DENV–vsRNA-5 and NS1 interaction. (A) Northern blot analysis of RNA from Aag2 cells cotransfected with the synthesized NS1 target ssRNA and control or vsRNA-5 mimic using a specific probe to ssRNA. aae-miR-2940 was used as loading control. (B) RT-PCR analysis of NS1 transcript levels after cotransfection of Aag2 cells with pIZ/NS1-full and control or vsRNA-5 mimics in two experimental replicates. RPS17 amplification is shown as control. (C) Western blot analysis of NS1 protein levels after cotransfection of Aag2 cells with pIZ/NS1-full and control or vsRNA-5 mimics using a monoclonal antibody to NS1. A nonspecific protein (arrowhead) bound to the antibody shows equal loading of the samples.