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(A) Nucleotide sequence alignment to show conservation of DENV–vsRNA-5 among the four DENV serotypes. The accession numbers for the serotypes 1–4 are GU131786.1, M29095.1, JF808129.1, and KF041260.1, respectively. (B) RT-qPCR analysis of RNA from Aag2 cells transfected with vsRNA-5 mimics of DENV-1, -2, -3, and -4 followed by DENV-2 infection at 72 hpi. There are statistically significant differences between groups with different letters at P < 0.0001.
