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. 2014 Feb 3;111(7):2656–2661. doi: 10.1073/pnas.1323436111

Table 1.

Peptides used in these studies

MHCII	Protein	Peptide	Sequence^* Groove position (p) 123456789	Form (soluble or MHCII-linked)
I-A^g7	Insulin (B:9–23)	Natural	`SHLVEALYLVCGERG`	Soluble
I-A^g7	Insulin (B:9–23)	R1:RE	`HLREALYLVCEERG`	Linked
I-A^g7	Insulin (B:9–23)	R2:RE	`HLVRALYLVCGERG`	Linked
I-A^g7	Insulin (B:9–23)	R3:RE	`HLVERLYLVCGEEG`	Both
I-A^g7	Insulin (B:9–23)	R3:REss	`HLVERLYLVCGEEG-α62`^†	Linked
I-A^g7	Insulin (B:9–23)	R3:RGE	`HLVERLYLVCGGEG`	Soluble
I-A^g7	Insulin (B:9–23)	R3:RGEss	`HLVERLYLVCGGEG-α62`^†	Linked
I-A^g7	Insulin (B:9–23)	R3:E	`HLVEALYLVCGEEG`	Soluble
I-A^g7	Insulin (B:9–23)	R3:RAE	`VERLYLVAGEEG`	Linked
I-A^g7	Lysozyme	HEL	`MKRHGLDNYRGY`	Linked
I-A^g7	Chromogranin A	HRPI^‡	`HRPIWARMD`	Soluble
I-A^g7	Chromogranin A	ChgA^‡	`SRLGLWSRMD`	Linked
I-A^b	MHCII Eα	3K^¶	`ASFEAQKAKANKAVDKA`	Linked
na	Tetnus toxin	TT	`QYIKANSKFIGITE`	Soluble

na, not applicable.

^*

Boldcase indicates differences from the wild type peptide.

^{^†}

Disulfide linked to I-A^g7 (p6Cys to I-A^g7-α62Asn mutated to Cys).

^{^‡}

Peptide that mimics the Chromogranin A WE-14 peptide.

^{^¶}

Peptide from mouse MHCII Eα mutated at three positions to lysine.