Fig. 4.

Mechanism of turnover of Golgi-derived vesicles involves the autophagy–lysosomal system and is consistent with altered gene expression in the human brain. (A–C) Selective Golgi clearance involves the autophagy–lysosome system. (A) Cells coexpressing LRRK2 (red) and GUS (blue) stained for TGN46 (green) after treatment for 24 h with DMSO or 10 nM bafilomycin A1. A cell with only residual TGN46 is shown in Right. (B and C) Blinded counts of the proportions of Golgi phenotypes in cells treated with (B) bafilomycin A1 or (C) knockdown of Atg7. (D and E) Gene expression in the human brain. (D) Direct assay of rs708723 in Rab7L1 from the brain of an individual who was heterozygous at this marker using RNA-Seq. (E) Density plots for the proportion of reads with the C allele for RNA-Seq (red) and exome data for DNA (blue) in brains from individuals heterozygous for rs708723. There is departure from the expected proportion of 0.5 (black vertical line) for RNA-Seq data.