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. 2014 Feb 3;111(7):2554–2559. doi: 10.1073/pnas.1312570111

Fig. 4.

Fig. 4.

LKB1 loss promotes HIF-1α protein expression under normoxic conditions. (A) Immunoblot for HIF-1α protein expression in whole-cell lysates from control (Cre−) or LKB1-null (Cre+) MEFs grown under 20% O2. (B) Relative expression of hif1a mRNA by control (Cre−, open bar) or LKB1-null (Cre+, filled bar) MEFs as determined by qPCR. Data were expressed relative to actin mRNA levels for triplicate samples and normalized relative to control (Cre−) cells. (C) Immunoblot of HIF-1α protein in lysates from A549/Vec or A549/LKB1 cells grown under 20% O2. (D) Relative expression of aldoa, ldha, and pdk1 mRNA levels in control (Cre-, open bar) or LKB1-null (Cre+, filled bar) MEFs as determined by qPCR. (E) Immunoblot for Aldolase, PDK1, and LDHA expression in lysates from cells as in D.