Fig. 5.

Preferential distribution of platelet-derived IDUA in liver and spleen was associated with clearance of senile platelets. Five months after transplantation, organs were collected from MPS I mice that were transplanted with WT marrow (MPS/WT) or MPS/KIiG marrow with 1.7 ± 1.1% transgene frequency (MPS/2%GT). (A) Enzyme activities in plasma and organs of perfused animals (n = 5–7 per group). **P < 0.001. (B) GAG levels in spleen, liver, heart, and brain (n = 5–7 per group). **P < 0.01; *P < 0.05. (C) Representative images from immune-fluorescence staining of liver and spleen sections. Samples were stained with antibodies against GFP (green) and CD68 (red) and counterstained with DAPI for nuclei (blue). Areas within yellow Insets are amplified to show GFP-containing hepatocytes (arrowhead), Kupffer cells (arrow), or spleen macrophages. MZM, marginal zone macrophages; R, red pulp; W, white pulp. (Scale bars, 100 μm.)