A) Conditioning (●) and test (○) amplitudes before and after cotinine (SQ) administration. The 0.10, 0.33 and 1 mg/kg doses produced significant increases in the conditioning amplitude. Asterisks indicate time points following cotinine administration where a significant effect, as determined by Fisher’s PLSD, on the conditioning amplitude were found as compared to an averaged baseline (b) for the corresponding dose. B) TC ratios before and after cotinine administration. There were no significant effects of cotinine on TC ratio at any dose tested. Data are mean ± SEM. For 0.33 mg/kg, n = 4; for all other doses, n = 8. *p < 0.05, **p < 0.01.