Figure 3. Silencing of FANCF enhanced DNA damage in breast cancer cells. A, Single-cell gel electrophoresis (comet assay) showed detectable comet tails when visualized under a fluorescent microscope, indicative of DNA damage. B, Quantification of DNA fragmentation in the indicated cell lines. Data are reported as means±SD of three independent experiments in triplicate. ANOVA followed by the post hoc test was used for statistical analyses.