Table 1.

Characteristics of PCR primers sequence, target fragment length and annealing temperature used for genotyping MTHFR polymorphisms

Polymorphisms sites		Primers sequence (5′ → 3′)	Fragment length	Annealing temperature
MTHFR C677T	F	AGTCCCTGTGGTCTCTTCATC	387 bp	56°C
	R	GGAGATCTGGGAAGAACTCAG
MTHFR A1298C	F	CTTTGGGGAGCTGAAGGACTACTAC	168 bp	55°C
	R	CACTTTGTGACCATTCCGGTTTG