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. 2014 Jan 18;15(1):41. doi: 10.1186/1471-2164-15-41

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© Ma et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Genome editing activity of TALEN-702. (A) In vivo analysis of TALEN activity in HEK 293 T cells using a luciferase reporter system. The entire TALEN-702 recognition site was cloned into a pSSA-Luc vector and transfected into HEK 293 T cell with (TALEN-702) or without (control) in vitro transcribed TALEN-702 mRNA. Luciferase activity was detected 24 hours post transfection. (B) Transient assay of TALEN activity in silkworm embryos using T7EI assay. PCR was performed using primer 702 F-883 and 702R-611 using genomic DNA from un-injected embryos (lane 2 and 3) or injected embryos (lane 4 and 5). M represents DNA ladders, the letters on the left indicate length of each band.