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. 2014 Jan 18;15(1):41. doi: 10.1186/1471-2164-15-41

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© Ma et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Detection of large chromosomal structural variation. (A and B) PCR was performed using primer set to detect chromosomal deletion (lane 4), duplication (lane 5), and inversions (lane 6 and 7) from genomic DNA of un-injected embryos (A) and injected embryos (B). For comparison, primer sets to amplify the flanking regions of TALEN-702 site (lane 2) and TALEN-B2 site (lane 3) were also used. M represents DNA ladders, the letters on the left indicate length of each band. (C) Sequences of the PCR products. The wild-type sequence is shown at the top with the full TALEN-B2 site in blue and the TALEN-702 site in red. The TALEN recognition sites are underlined, and deletions are indicated by dashed lines.