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Isolation and analysis of DH4–FB1 from the FUM7 mutant. (A) HPLC analysis of crude extract; (B) HPLC analysis of the initially purified DH4–FB1; (C) HPLC analysis of the isomerized DH4–FB1; (D) MS analysis of the initially purified DH4–FB1; and (E) MS analysis of the isomerized DH4–FB1. A proposed cis–trans stereoisomerization of DH4–FB1 is included.
