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. Author manuscript; available in PMC: 2014 Dec 18.
Published in final edited form as: Neuron. 2013 Dec 18;80(6):1421–1437. doi: 10.1016/j.neuron.2013.10.050

Figure 3. GLR-1 is redistributed between synapses.

Figure 3

(A) Photoactivation of GLR-1∷PAGFP expressed in AVA before and after UV photoactivation (blue, dashed box) with the corresponding kymograph. Arrowheads show anterograde (blue arrowheads) and retrograde (inset, red arrowheads) departure of a GLR-1 vesicle from converted synaptic puncta. (B–E) All confocal images were taken before, immediately after, or 4 hours after photoconversion or photoactivation. (B) Schematic of GLR-1∷Dendra2 photoconversion in the cell body (top). Images of GLR-1∷Dendra2 in the cell body and in the distal processes (tail). Red arrowheads highlight synapses that received photoconverted GLR-1∷Dendra2 from the cell body. (C and D) Images of GLR-1∷Dendra2 after photoconversion of total fluorescence (C) or puncta fluorescence only (D) in the proximal processes (only the red signal is shown). Red arrows indicate the appearance of photoconverted GLR-1∷Dendra2 at distal synapses (red and green signal shown). (E) Images of GLR-1∷PAGFP before and after photoactivation. Red arrows indicate the appearance of photoactivated GLR-1∷PAGFP at proximal synapses.

Scale bars represent 5 µm.

See also Figure S3.