Skip to main content
. Author manuscript; available in PMC: 2014 Dec 18.
Published in final edited form as: Neuron. 2013 Dec 18;80(6):1421–1437. doi: 10.1016/j.neuron.2013.10.050

Figure 5. Delivery and removal of synaptic GLR-1 is mediated by UNC-116/KIF5.

Figure 5

(A) Confocal images of GLR-1∷GFP puncta in the proximal region of the AVA processes in various transgenic worms. (B) Quantification of GLR-1∷GFP synaptic puncta fluorescence normalized to WT. For all genotypes, n≥10 worms. (C) Confocal images of GLR-1∷Dendra2 synaptic puncta (red signal only) before and after photoconversion. Scale bar represents 1 µm. (D) Quantification of the red signal remaining 4 hours after photoconversion, n=15 puncta per genotype. (E and F) Images (E) and quantification (F) of synaptic GLR-1∷GFP puncta in AVA normalized to the proximal region of WT, n=10 worms. Scale bar represents 5 µm. (G) Cartoon schematic of the distal photobleach experiment. (H) GLR-1∷GFP images before, immediately after, and 4 hours after photobleaching in the regions indicated in (G). Scale bar represents 5 µm. (I) Linescans of GLR-1∷GFP fluorescence intensity in the distal half of AVA before and after photobleaching.

* p<0.05, ** p<0.01, *** p<0.001. Error bars indicate SEM.

See also Figures S4, S5, S6, S7 and S8.