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. 2014 Feb 6;2014:875105. doi: 10.1155/2014/875105

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Copyright © 2014 H. Alkhouri et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

The effects of human lung mast cell products released early after activation on asthmatic (A) and non-asthmatic (NA) ASM cell CXCL10 production. Mast cells (MC) were isolated from human lung and immediately activated with IgE/anti-IgE in culture medium (DMEM + 10% FBS) and the culture supernatants collected after 2 hours (2 h MC SN). The MC SN were added at 20% and 40% v/v to ASM cells stimulated with IFNγ and CXCL10 levels were quantified after 48 hours stimulation using ELISA. ^∗ P < 0.05 compared with the DMEM + 10% FBS control (a) or FBS-IFNγ control ((b) and (c)), ANOVA.