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. 2014 Feb 15;14:48. doi: 10.1186/1471-2229-14-48

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Copyright © 2014 Xu et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Isolation of ecr2-1. (A) and (B) Genetic analyses and chi-square test of ecr2-1. Numbers are individuals scored in the F2 generation. Other: other phenotype that is not ecr2-1 ctr1-10 growth inhibition phenotype. (C)ECR2 is mapped to a region spanning the centromere at chromosome 2, and the BAC clones are indicated. (D) Results for 3 independent test crosses for ecr2-1 with ctr1-10. Phenotype (E) and ethylene dose–response assay for the hypocotyl measurement (F) and relative hypocotyl length (G) of etiolated wild-type (Col-0) and ecr2-1 seedlings. The dotted line indicates 50% growth inhibition for (G); P values for Student’s t test are indicated. (H) Rosette phenotype of ecr2-1. (I) Ethylene evolution of light-grown seedlings (5 days after germination). Data are mean±SD of 3 independent measurements.