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. Author manuscript; available in PMC: 2014 Apr 17.
Published in final edited form as: Nature. 2013 Aug 28;502(7471):377–380. doi: 10.1038/nature12508

Figure 3. GATM knockdown attenuated sterol-mediated induction in expression of SREBP-responsive genes.

Figure 3

(a) Changes in transcript concentrations following sterol depletion via 24-hr exposure to lipoprotein deficient serum (LPDS)-containing media vs. standard FBS-containing media in hepatocyte-derived HepG2 (left, N=12) and Huh7 (right, N=12) cell lines. Asterisk indicates P<0.05 for the comparison of GATM versus NTC siRNA treated cells. (b) Fold changes in media accumulation of apolipoprotein B (ApoB) and apolipoprotein AI (ApoAI) following gene knockdown with GATM versus (NTC) siRNA in HepG2 cells (left, N=6-10) or Huh7 (right, N=4-6) cells under standard culture conditions. Error bars represent SEM.