Figure 8. Altering the terminal Ala-Ala motif of σ^AntA results in higher expression of σ^AntA targets.

The ΔantA null mutant was complemented with either a wild-type copy of antA or a variant of antA encoding A172D and A173D point mutations. After 42 h of growth, transcription of σ^AntA-targets in the ΔantA/antA-DD strain were significantly greater than both the wild-type and ΔantA/antA-AA strains, suggesting the terminal Ala-Ala motif modulates stability and/or activity of σ^AntA. ** and *** denote that values reported are statistically significantly different with a P value < 0.01 and 0.001 in a Student’s T-test, respectively.