Figure 8.

DNA amplification of the 3,426-bp fragment of BRCA1 exon 11 after cellular treatment with the ruthenium(II) polypyridyl complexes. MCF-7 (A), MDA-MB-231 (B), and HCC1937 (C) cells were treated with various concentrations of 1 and 2 at 37°C for 24 h before genomic DNA was isolated. Genomic DNA was then amplified with forward/reverse primers for the 3,426-bp fragment of the BRCA1 gene in a PCR reaction mixture for 30 cycles. PCR products were electrophoresed on 1% agarose gel. The gel was stained with ethidium bromide and visualized under UV illumination. Amplification products were quantified as described in the materials and methods section. The amount of DNA amplification (%) was plotted as a function of the drug concentration. The mean ± the standard error of experiments realized in duplicate is plotted. Statistical significance differences from the untreated control are indicated by * p < 0.01.