Figure 2. ARHI induces autophagy in ovarian cancer cells. (A) Expression of ARHI in the ovarian cancer dormancy-recurrence in-vitro model as measured by real-time PCR. Top: SKOv3-ARHI; bottom: Hey-ARHI. (B) Increased cleavage of LC3-I to LC3-II and higher ARHI expression were shown in the dormancy group, whereas the withdrawal of DOX resulted in decreased LC3-II and ARHI expression in SKOv3-ARHI (C) The percentage of autophagic cells was measured by acridine orange staining and flow cytometry. FL3H detects red fluorescence (650 nm), and FL1H detects blue fluorescence (488 nm). Dot plots that show increased autophage formation in the dormancy (VEGF+DOX treatment) group and decreased autophage formation after the transition of dormancy to recurrence (VEGF + DOX withdrawn). *Compared with the VEGF control group, P < 0.05, **Compared with the VEGF control group, P < 0.01; ^#Compared with the dormancy group, P < 0.01, ^##Compared with the dormancy group, P < 0.01.