Table 2.
Details of RT-LAMP and RT-PCR primers designed for detection of NA gene sequences of N9 subtype influenza viruses.
| Primer namea | Genome position | Length(s) | Sequences (5′ to 3′) |
|---|---|---|---|
| N9-F3 | 18-mer | GTTTCATGCGACCCAGAT | |
| N9-B3 | 20-mer | CATGGCAACTAGTACTTGAC | |
| N9-FIP | 41-mer | TTCCGTTTGAGTGTTTCCCTCGAATGCAGGTTCTATGCTCT | |
| N9-BIP | 39-mer | CAGTATCGCGCCCTGATAAGCCACCCAATGCATTCCACC | |
| N9-LB | 23-mer | TGGCCACTATCATCACCGCCCAC | |
| N9-LF | 18-mer | TGATTGTTGTTCCTTGGC | |
| N9 F | 22-mer | ATAATGAAACAAACATCACCAA | |
| N9 R | 22-mer | AGCATAGAACCTGCATTCATCT |
aThe primers of N9-F3, N9-B3, N9-FIP, N9-BIP, N9-LB, and N9-LF were for RT-LAMP Primers. N9-F and N9-R were applied to RT-PCR.