Skip to main content
. 2014 Feb 24;9(2):e89367. doi: 10.1371/journal.pone.0089367

Figure 1. T. whipplei survives and replicates in a Lamp-1- but not cathepsin D-positive compartment within BMDMs.

Figure 1

(A) BMDMs were incubated with T. whipplei (bacterium-to-cell ratio of 50∶1) for 4 hours (day 0). Cells were then washed to remove free bacteria and incubated for additional periods (days). The copy number of bacterial DNA was determined by qRT-PCR. The results are expressed as the mean ± SD from 3 experiments (*p<0.05). (B–D) The colocalisation of T whipplei with either (B) Lamp-1 or (C) cathepsin D was analysed in BMDMs by immunofluorescence and confocal microscopy. The percentage of T whipplei colocalising with either Lamp-1 or cathepsin D was quantified over the time (D). The results are expressed as the mean ± SD from 3 experiments (*p<0.05). The scale bars indicate 5 µm.