Table 1. Pathogenic canine leptospiral strains detectable by the Leptospira spp. lig FRET-PCR as indicated by sequence matches of primers and probes.

Species	Serogroup	Serovar	Strain	Gene accession #a
L. interrogans	Icterohaemorrhagiae	Copenhageni	Fiocruz L1-130	AE016823.1
L. interrogans	Icterohaemorrhagiae	Lai	56601	AE010300.2
L. interrogans	Icterohaemorrhagiae	Lai	IPAV	CP001221.1
L. interrogans	Pomona	Pomona	Kennewicki PO-06047	EU700267.1
L. interrogans	Pomona	Pomona	Kennewicki PO-06047	EU700268.1
L. interrogans	Pomona	Pomona	Kennewicki Cornell	AF534640.1
L. interrogans	Pomona	Pomona	Kennewicki	AF368236.1
L. interrogans	Pomona	Pomona	Kennewicki	U95056
L. interrogans	Pomona	Pomona	pLPLIGB	FJ030916.1
L. interrogans	Pomona	Pomona	pLPLIGA	FJ030617.1
L. interrogans	Canicola	Canicola	Kito	EU700267.1
L. interrogans	Canicola	Canicola	Kito	EU700268.1
L. interrogans	Canicola	Canicola	Hond Ultrecht IV	EU289225.1
L. interrogans	ND	ND	FPW1039	AKWR02000178.1
L. interrogans	ND	Manilae	UP-MMC-NIID	AB098516.1
L. interrogans	ND	Manilae	UP-MMC-NIID	AB098517.1
L. kirschneri	Grippotyphosa	Grippotyphosa	RM52	EF517920.1
L. kirschneri	Grippotyphosa	Grippotyphosa	Moskva V	AY190126.2

^aEach of the four oligonucleotides in the lig FRET-PCR was used in nucleotide megablast searches (optimized for highly similar sequences) against all accessioned sequences in GenBank, and all matched to these 18 strains of pathogenic leptospires with 0–1 nucleotide mismatch.