Table 1.
For investigating the AMH and AMHRII polymorphisms, genotypes were decided using Taqman allelic discrimination assays
| Gene | Base pair change | Position (aa change) | Primers | Base pair | Tm (C0) |
|---|---|---|---|---|---|
| AMH | 146 T>G | Exon1 | F:ACCAGTGGCCTCATCTTCC | 152 | 59 |
| R:AGGAAGGCCTGCTCATAGG | |||||
| AMHR-II | −482 A>G | Promoter | F:GGTAACCTCTAATATGGGCTGTG | 150 | 59 |
| R:TCTCAGGAGGAAACCAATGTG | |||||
| AMHR-II | IVS1+149 T>A | Intron 1 | F:CCCTTTGGAAGAGTGGTGAG | 196 | 56 |
| (−) | R:AGGTGGGAGTGAATGCAGAG | ||||
| AMHR-II | IVS5-6 C>T | Intron 5 | F:AGCTGTGTTTCTCCCAGGTG | 197 | 56 |
| (−) | R:ATGGCAACCAGTTTTCCTTG | ||||
| AMHR-II | IVS10+77 A>G | Intron 10 | F:AAGAGGCCTAGGCTGTTGGT | 241 | 60 |
| (−) | R:CAAGGTTGAGCAGGAGGAAG |