Fig. 1.

Fluorescent microscope photographs of in vitro mature goat oocytes. A Oocytes were stained with Cell tracker Blue (a-f) and 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) (g-i) to detect intracellular levels of glutathione (GSH) and reactive oxygen species (ROS), respectively. Mature oocytes derived from the maturation medium treated with 0 μM (a and g), 0.1 μM (b and h), 0.25 μM (c and i), 0.5 μM (d and j), 2.0 μM (e and k), 5.0 μM (f and l). B Effect of resveratrol treatment in IVM medium on intracellular GSH and ROS levels in mature goat oocytes. Within each group (GSH and ROS) different superscript letters are significantly (P < 0.05) different. Experiment was replicated three times