Fig. 2.

HspA1A facilitates repair of DNA damage induced by Benzo[a]pyrene (BaP) and Benzo(a)pyrene diol epoxide (BPDE). a The 16HBE cells were treated with 16 μM BaP for 2 h and recovered for 0, 2, 4, 8 and 24 h. Results are expressed as mean ± SD from three independent experiments. **p < 0.01 compared with the control at 0 h; ‡p < 0.01 compared with the control in the first 2 h of repair. b The firefly luciferase reporter plasmids PCMVluc were incubated with various doses of BPDE for 3 h at room temperature before they were transfected into 16HBE cells. After 48 h of transfection, the luciferase activities of the cell lysates were analyzed. DNA repair capacity was calculated as the percentage of luciferase activity from the damaged reporter to that of the unmodified reporter. Results are expressed as mean ± SD from three independent experiments. *p < 0.05, **p < 0.01 compared with the corresponding control