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. 2014 Feb 24;9(2):e89760. doi: 10.1371/journal.pone.0089760

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© 2014 Sterken et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Shows the outline of the experiment, worms were synchronized and 26h thereafter exposed to OrV. 72h past bleaching the worms have laid eggs, at this point the experiment progresses in two parts (indicated by the triangles and squares), either re-synchronized and re-infected populations (triangles) or populations that were only transferred (squares). (B) The outcome of the experiments (n = 6) for the re-infected populations. Only in N2 there was a significant reduction in virus titer in the pre-exposed populations compared to the naive populations (ANOVA, P≤0.05). (C) The outcome of the experiment where the populations were transferred (so no re-infection). Also here only N2 showed a significant reduction in virus titer in the following generations compared to the first generation (ANOVA, P≤0.01).