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. 2014 Feb 24;9(2):e89811. doi: 10.1371/journal.pone.0089811

Figure 1. TCDD induces premature senescence in PC12 neuronal cells.

Figure 1

(A) Upper panel: PC12 cells were treated with DMSO or 1, 10, 50 and 100 nM TCDD for 72 h and then subjected to an SA-β-Gal staining assay. Lower panel: PC12 cells were treated with 50 nM TCDD for 0, 24, 48, 72 and 96 h and then subjected to an SA-β-Gal staining assay. (B) The number of SA-β-Gal positive cells in each group from Fig. 1A was counted and listed (* p<0.05, significantly different from the DMSO-treated group). (C) PC12 cells were treated with DMSO or 50 nM TCDD for 72 h and then immunostained with FITC–phalloidin to visualize F-actin. (D) PC12 cells were treated with DMSO or 50 nM TCDD for 72 h and were immunostained with a γ-H2AX antibody.