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. 2014 Feb 24;9(2):e89811. doi: 10.1371/journal.pone.0089811

Figure 6. TCDD-induced oxidative DNA and lipid damage were attenuated by the ROS scavenger NAC.

Figure 6

(A) ROS fluorescence was examined in PC12 cells that had been treated with DMSO, 50 nM TCDD or 50 nM TCDD plus NAC. (B) Flow cytometric analysis of ROS fluorescence in each group. (C) PC12 cells treated with DMSO, 50 nM TCDD or 50 nM TCDD plus NAC for 72 h were immunostained with an anti-8-oxo-dG antibody. (D) Determination of lipid oxidation by analyzing the MDA content per milligram of total protein of PC12 cells after DMSO, 50 nM TCDD or 50 nM TCDD plus NAC treatment for 72 h (* p<0.05, statistically significant difference from the control group; # p<0.05, statistically significant difference from the TCDD-treated group).