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. 2014 Feb 24;9(2):e89811. doi: 10.1371/journal.pone.0089811

Figure 7. NAC inhibited TCDD-induced senescence in PC12 neuronal cells.

Figure 7

(A) PC12 cells were pre-treated with DMSO, 50 nM TCDD, or 50 nM TCDD plus 5 mM NAC for 72 h before SA-β-Gal staining was performed. (B) The number of positive cells out of every 500 cells was counted and presented (* and # p<0.05, statistically significant difference from the control group; # p<0.05, statistically significant difference from the TCDD-treated group). (C) Western blot analysis of the senescence marker proteins, p16 and p21, after the cells had been treated with DMSO, 50 nM TCDD, or 50 nM TCDD and NAC for 72 h. (D) Statistical analysis of the densitometric evaluation of the three independent experiments (* p<0.05, statistically significant difference from the control group; # p<0.05, statistically significant difference from the TCDD-treated group).