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. 2014 Feb 24;9(2):e89811. doi: 10.1371/journal.pone.0089811

Figure 8. TCDD induces premature senescence in the human neuroblastoma SH-SY5Y cell line.

Figure 8

(A) SH-SY5Y cells were incubated with DMSO, 10 nM TCDD (B), 30 nM TCDD, 50 nM TCDD or 50 nM TCDD combined with 5 mM NAC. Seventy-two hours later, the cells were subjected to an SA-β-Gal assay. The percentage of β-Gal-positive cells in each group was counted and presented (* p<0.05, statistically significant difference from the control group; # p<0.05, statistically significant difference from the 50 nM TCDD-treated group). (B) TCDD exposure altered the expression of senescence marker proteins in an ROS-dependent manner in SH-SY5Y cells. SH-SY5Y cells were exposed to DMSO, 50 nM TCDD or 50 nM TCDD+5 mM NAC and then subjected to western blot analyses to determine p16, p21 and p-Rb expression. (C) Quantitative analysis of the intensity of protein expression relative to GAPDH in the indicated groups (* p<0.05, statistically significant difference from the DMSO-treated group; # p<0.05, statistically significant difference from the 50 nM TCDD-treated group).