Figure 4.

Insulin activates the PI3K/Akt in melanoma cells.

Notes: Whole cell lysates from B16 and Mel-RMu cells with or without treatment with insulin (250 nM) for 15 minutes were subjected to Western blot analysis of phosphorylated Akt, Akt, and GAPDH (as a loading control). The data shown are representative of three individual experiments.

Abbreviations: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Akt, protein kinase B.