Figure 1. Gel image of amplicons produced using varying extract volumes.

Annotated gel image of round two PCR products from nested PCR using varying volumes of diluted DNA extracts in the preceding round one reaction. The products for all seven isolate extracts at the highest two dilutions are shown. Wells to the left of the image contain 10⁻⁷ dilution extracts (600 ag/µL) and wells adjacent to the molecular weight ladder contain 10⁻⁸ dilution extracts (60 ag/µL). The extract/reaction volumes (µL) are indicated on the right of the image. Extract numbers refer to the order in which the isolates are listed in Table S1. ML = Molecular ladder.